White Paper — Sponsored Content

In-line Control of CO2 Accumulation for Improved Cell Culture Processes

Cell Sensor - METTLER TOLEDO Group - ISPE Pharmaceutical Engineering


The importance of preventing dissolved CO₂ accumulation

Controlling dissolved CO₂ measurement in mammalian cell culture processes is growing in acceptance.

dCO₂ concentration in the human body is 5% and when growing animal cells ex-vivo it is necessary to maintain this level as a minimum concentration. In an ex-vivo culture model, for example in a bioreactor, measuring and monitoring dCO2 is considered critical for several reasons. dCO2 accumulation can have a substantial negative effect on cell growth as well as on productivity and the glycosylation of the targeted protein of interest.

Blood gas analyzers do not provide sufficient information

Controlling dissolved carbon dioxide accumulation in cell culture by sparging with air or oxygen is common, particularly on production-scale bioreactors. To monitor dCO₂, samples are normally taken for measurement in an off-line blood gas analyzer (BGA). This method is not without its issues: infrequent sampling does not provide continuous insight, and temperature changes and time delay between samples being taken and measured can affect dCO₂ level. These issues are not just confined to BGAs. Any off-line or at-line method can be subject to influences that prevent a true picture of dCO₂ levels being established.

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