Containment (Containmt) is the action of confining within a defined space a microbiological agent or other entity that is being cultured, stored, manipulated, transported, or destroyed to prevent or limit its contact with people and the environment. Methods to achieve containment include physical and biological barriers and inactivation using physical or chemical means.1.Primary Containment. Addresses the protection of personnel and the immediate laboratory environment from exposure to infectious agents. It involves using closed containers or biological safety cabinets along with secure operating procedures.
2. Secondary Containment. A system of containment that prevents the escape of infectious agents into the environment external to the laboratory. It involves the use of rooms with specially designed air handling, the existence of airlocks and sterilizers for the exit of materials, and secure operating procedures. In many cases, it may add to the effectiveness of primary containment. The three main elements of containment include laboratory practice and technique (the most important aspect), safety equipment (primary barriers), and facility design (secondary barriers).