ISPE Glossary of Pharmaceutical and Biotechnology Terminology

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A method for cloning large regions of a chromosome. Starting from a known site, a gene library is screened for clones that hybridize to DNA probes taken from the ends of the first clone. These clones are then isolated, and their ends used to screen the library again. These clones are then isolated and their ends used, and so on.

Publication Source: Food and Agricultural Organization of the United Nations (FAO)
Publication Date: 1999

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